Publications - Oliver Griesbeck



Schnaitmann C, Haikala V, Abraham E, Oberhauser V, Thestrup T, Griesbeck O, Reiff DF (2017). Parallel color-opponent pathways originate in Drosophila photoreceptor terminals. Cell, in press


Osswald M, Jung E, Sahm F, Solecki G, Blaes J,  Weil S, Venkataramani V, Horstmann H, Wiestler B, Syed M, Huang L, Ratcliff M, Jazi KK, Kurz FT, Schmenger T, Lemke D, Gömmel M, Pauli MA, Liao Y, Häring P, Pusch S, Herl V, Steinhäusser C, Krunic D, Jarahian M, Miletic H, Berghoff A, Griesbeck O, Garaschuk O, Preusser M, Weiss S, Liu H, Heiland S, Platten M, Huber PE, Kuner T, Deimling A, Wick W,  Winkler F (2015). Brain tumor cells interconnect to a functional and resistant network. Nature 528: 93-98

Gopal S, Søgaard P, Multhaupt HA, Pataki C, Okina E, Xian X, Pederson ME, Stevens T, Griesbeck O, Park PW, Pocock R, Couchman JR (2015). Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels. J Cell Biol 210:1199-211

Kovalchuk Y, Homma R, Liang Y, Maslyukov A, Hermes M, Thestrup T, Griesbeck O, Ninkovic Y, Cohen LB, Garaschuk O (2015). Rapid maturation of odorant response properties in migrating adult-born neurons of the mouse olfactory bulb. Nature Communications 6: 6349

Litzlbauer J, Ng D, Fabritius A, Schifferer M, Thestrup T, Griesbeck O (2015). Large scale bacterial colony screening of diversified FRET biosensor libraries. PloS One 10(6):e0119860

Siffrin V, Birkenstock J ,  Luchtmann D ,  Gollan R, Baumgart J , Niesner RA, Griesbeck O, Zipp F  (2015). FRET based ratiometric Ca2+ imaging to investigate immune-mediated neuronal and axonal damage processes in experimental autoimmune encephalomyelitis.  J. Neurosci Methods 249:8-15

Thestrup T, Griesbeck O (2015). Protein-based calcium sensors. In: Optical Probes in Biology, CRC Press, Taylor & Francis Group LLC


Williams PR, Sorbara CD, Mahler CF, Schuhmacher AM, Griesbeck O, Kerscheinsteiner T, Misgeld T (2014). A recoverable state of axon injury persists for several hours after spinal cord contusion in vivo. Nature Communications 5: 5683

Rose T, Goltstein P, Portugues R, Griesbeck O (2014). Putting a finishing touch on GECIs. Front Mol Neurosci 7: 88

Breckwoldt M, Pfister F, Bradley PM, Marinkovic P, Williams P, Brill MS, Plomer B, Schmalz A, Godinho L, Bareyre FM, Naumann R, Griesbeck O, Dick TP, Kerschensteiner M, Misgeld T (2014). Multi-parametric optical analysis of mitochondrial redox signals during neuronal physiology and pathology in vivo. Nature Medicine 20: 555-560

Grimm C, Holdt LM, Chen CC, Hassan S, Butz E, Spahn S, Northoff B, Müller C, Castongay J, Luber CA, Kissing S, Schröder B, Fendel C, Klugbauer N, Griesbeck O, Moser M, Bracher F, Teupser D, Mann M, Haas A, Saftig P, Biel M, Wahl-Schott C (2014). Two-pore channel 2 deficient mice are prone to hyperlipoproteinemia and hepatic cholesterol overload. Nature Communications 5: 4699

Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Witte G, Chen TW, Becker S, Laukat Y, Geiger A, Allen T, Kim DS, Garaschuk O, Griesinger C, Griesbeck O (2014). Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T-lymphocytes. Nature Methods 11: 175-182


Mues M, Bartholomäus I, Thestrup T, Griesbeck O, Wekerle H, Kawakami N, Krishnamoorthy G (2013). Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator. Nature Medicine 19: 778-783

Aramuni G, Griesbeck O (2013). Chronic calcium imaging in neuronal development and disease. Exp Neurol 242: 50-56


Schifferer M, Griesbeck O (2012). A dynamic FRET reporter of gene expression improved by functional screening. J Am Chem Soc 134: 15185-15188

Direnberger S, Mues M, Micale V, Wotjak CT, Scharr CA, Schubert KM, Dietzel S, Wahl-Schott C, Biel M, Krishnamoorthy G, Griesbeck O (2012). Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. Nature Communications 3:1031

Wei T, Schubert T, Paquet-Durand F, Tanimoto N, Chang L, Koeppen K, Ott T, Griesbeck O, Seeliger M, Euler T, Wissinger B (2012). Light-driven calcium signals in mouse cone photoreceptors. J Neurosci 32: 6981-6994

Geiger A, Russo L, Gensch T, Thestrup T, Becker S, Hopfner KP, Griesinger C, Witte G, Griesbeck O (2012). Correlating calcium binding, FRET and conformational change in the biosensor TN-XXL. Biophys J 102: 2401-2410

Tay LH, Dick IE, Tadross MR, Yang W, Mank M, Griesbeck O, Yue DT (2012). Nanodomain Ca2+ of Ca2+ channels detected by a tethered genetically encoded Ca2+ sensor. Nature Communications 3: 778

Aramuni G, Griesbeck O (2012). Chronic calcium imaging in neuronal development and disease. Exp Neurol, Feb 21. [Epub ahead of print]

Looger LL, Griesbeck O (2012). Genetically encoded neural activity indicators. Curr Opin Neurobiol, 22: 18-23

Fink S, Kovalchuk Y, Homma R, Schwendele B, Direnberger S, Cohen LB, Griesbeck O, Garaschuk O (2012). In vivo functional imaging of the olfactory bulb at single cell resolution. Neuronal Network Analysis, Neuromethods Series 67, Humana Press ISBN 978-1-61779-632-6


Faumont S, Rondeau G, Thiele TR, Lawton KJ, McCormick KE, Sottile M, Griesbeck O, Heckscher ES, Roberts WM, Doe CQ, Lockery SR (2011). A lens-free optomechanical system for virtual navigation and calcium imaging from identified neurons in freely moving Caenorhabditis elegans. PloS One, 6: e24666

Ng D, Fromherz P (2011). Genetic targeting of a voltage-sensitive dye by enzymatic activation of phosphonooxymethyl-ammonium derivative. ACS Chem Biol, 6: 444-451

Zhan K, Xie H, Gall J, Ma ML, Griesbeck O, Salehi A, Rao J (2011). Real-time imaging of Rab5 activity using a pre-quenched biosensor. ACS Chem Biol, 6: 692-699


Siffrin V, Radbruch H, Glumm R, Niesner R, Lehnhardt S, Herz J, Leuenberger T, Paterka M, Lehmann SM, Rinnenthal JL, Laube G, Luche H, Fehling H-J, Griesbeck O, Zipp F (2010). In vivo imaging of partially reversible TH17-induced neuronal dysfunction in the course of encephalomyelitis. Immunity, 33: 424-436

Friedrich MW, Aramuni G, Mank M, MacKinnon J, Griesbeck O (2010). Imaging CREB activation in living cells. J Biol Chem, 285: 23285-23295

Reiff DF, Plett J, Mank M, Griesbeck O, Borst A (2010). Visualizing retinotopic half-wave rectified input to the motion detection circuitry of Drosophila. Nature Neurosci, 13: 973-978

Nguyen Q-T, Schroeder LF, Mank M, Muller A, Taylor PW, Griesbeck O, Kleinfeld D (2010). An in vivo biosensor for neurotransmitter release and exogenous receptor activity. Nature Neurosci, 13: 127-132


Griessmeier K, Cuny H, Rötzer K, Griesbeck O, Harz H, Biel M, Wahl-Schott C (2009). Calmodulin is a functional regulator of Cav1.4 L-type Ca2+ channels. J Biol Chem 284: 29809-29816

Hou BH, Takanaga H, Griesbeck O, Frommer WB (2009). Osmotic induction of calcium accumulation in human embryonic kidney cells detected with a high sensivity FRET calcium sensor. Cell Calcium 46: 130-135

Zong X, Schieder M, Cuny H, Rötzer K, Griesbeck O, Harz H, Gruner C, Biel M, Wahl-Schott C (2009). The two pore channel TPCN2 mediates NAADP-dependent CA2+ release from lysosomal stores. Pflügers Arch 458: 891-899

Schifferer M, Griesbeck O (2009). Application of aptamers and autofluorescent proteins for RNA visualization. Integr Biol 1: 499-505

Garaschuk O, Griesbeck O (2009). Monitoring calcium levels with genetically encoded indicators. In: Calcium Measurement Methods, vol. 43. Eds: A Verkhratsky, OH Petersen. Humana Press, Springer

Ducros M, Moreaux L, Bradley J, Tiret P, Griesbeck O, Charpak S (2009). Spectral unmixing: Analysis of performance in the olfactory bulb in vivo. PloS One 4: e4418


Mank M, Ferrão Santos A, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hübener M, Griesbeck O (2008). A genetically encoded calcium indicator for chronic in vivo two photon imaging. Nature Methods 5: 805-811

Hendel T, Mank M, Schnell B, Griesbeck O, Borst A, Reiff DF (2008). Fluorescence changes of genetic calcium indicators and OGB-1 correlated with neural activity and calcium in vivo and in vitro. J Neurosci 28: 7399-411.

Mank M, Griesbeck O (2008). Genetically encoded calcium indicators. Chem Rev 108: 1550-64


Tay LH, Griesbeck O, Yue DT (2007). Live-cell transforms between Ca 2+ transients and FRET responses for Troponin C-based Ca 2+ sensors. Biophys. J. 93: 4031-4040

Garaschuk O, Griesbeck O, Konnerth A (2007). Troponin C-based biosensors: a new family of genetically encoded indicators for in vivo calcium imaging in the nervous system. Cell Calcium 42: 351-61

Heim N, Garaschuk O, Friedrich MW,Mank M, Milos RI, Kovalchuk Y, Konnerth A, Griesbeck O (2007). Improved calcium imaging in transgenic mice expressing a troponin C–based biosensor. Nature Methods 4: 127-129.


Mank M, Reiff DF, Heim N, Friedrich MW, Borst A, Griesbeck O (2006). A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change. Biophys. J. 90: 17980-1796

Kleinfeld D, Griesbeck O (2005). From art to engineering? The rise of in vivo mammalian electrophysiology via genetically targeted labeling and nonlinear imaging. PloS Biology 3: 355

Griesbeck O (2004). Fluorescent proteins as sensors for cellular functions. Curr. Opin. Neurobiol. 14: 636-641

Hasan M T, Friedrich R W, Larkum M E, Petersen C C H, Euler T, Dubel J, Both M, Waters J, Bujard H, Griesbeck O, Tsien R Y, Miyawaki A, Denk W (2004). Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biology 2: 163

Heim N, Griesbeck O (2004). Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein. J. Biol. Chem. 279: 14280-14286.

Zapata Hommer O, Griesbeck O (2003). Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP. BMC Biotechnology 3: 5.


Griesbeck O, Baird G S, Campbell R E, Zacharias A, Tsien R Y (2001). Reducing the environmental sensitivity of yellow fluorescent protein: mechanism and application. J. Biol. Chem. 276: 29188-94

Miyawaki A, Griesbeck O, Heim R, Tsien R Y (1999). Dynamic and quantitative Ca2+-measurements using improved cameleons. Proc. Natl. Acad. Sci. USA 96: 2135-2140

Griesbeck O, Canossa M, Campana G, Gartner A, Hoener M C, Nawa H, Kolbeck R, Thoenen H (1999). Are there differences between the secretion characteristics of NGF and BDNF? Implications for the modulatory role of neurotrophins in activity-dependent neuronal plasticity. Micr. Res. Tech. 45: 262-275

Canossa M, Griesbeck O, Berninger B, Campana G, Kolbeck R, Thoenen H (1997). Neurotrophin release by neurotrophins: Implications for activity-dependent neuronal plasticity. Proc. Natl. Acad. Sci. USA 94: 13279-13286

Griesbeck O, Korte M, Staiger V, Bonhoeffer T, Thoenen H (1997). Rapid gene transfer into cultured hippocampal neurons and acute hippocampal slices using adenoviral vectors. Mol. Brain Res. 44: 171-177

Griesbeck O, Korte M, Staiger V, Gravel C, Carroll P, Bonhoeffer T, Thoenen H (1996). Combination of gene targeting and gene transfer by adenoviral vectors in the analysis of neurotrophin-mediated neuronal plasticity. Cold Spring Harbor Symp. Quant. Biol. 61: 77-83

Korte M, Griesbeck O, Gravel C, Carroll P, Staiger V, Thoenen H, Bonhoeffer T (1996). Virus-mediated gene transfer into CA1 region restores LTP in BDNF mutant mice. Proc. Natl. Acad. Sci USA 93: 12547-12552

Korte M, Staiger V, Griesbeck O, Thoenen H, Bonhoeffer T (1996). The involvement of brain-derived neurotrophic factor in hippocampal long-term potentiation revealed by gene targeting experiments. J. Physiol. (Paris) 90: 157-164

Griesbeck O, Parsadaninan A SH, Sendtner M, Thoenen H (1995). Expression of neurotrophins in skeletal muscle: Quantitative comparison and significance for motoneuron survival and maintenance of function. J. Neurosci. Res. 42: 21-33

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